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Anti - Flag Tag ( DYKDDDDK Tag ) Rabbit pAb
價(jià)格：
在線咨詢
貨號(hào)：
DRF10130
產(chǎn)品規(guī)格：
100 μL
Applications：
WB
Species：
--
購(gòu)買數(shù)量：
產(chǎn)品詳情
操作說(shuō)明
注意事項(xiàng)
Product Information
|
Description |
Flag Tag ( DYKDDDDK Tag ) rabbit polyclonal |
|
Synonyms |
DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to DYKDDDDK |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
-- |
1: 500-1: 1000 |
Ttransfected 293T cells |
Background
The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Images
|
Western blot analysis of FLAG Tag (GB11938) at dilution of 1: 1000 Lane 1: 293T cells transfected with empty vector Lane 2: 293T cells transfected with vector overexpressing Flag-tag fusion protein |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥萬(wàn)物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區(qū)黃山路602號(hào)合肥國(guó)家大學(xué)科技園A401室