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Anti -EIF2S2/EIF2B Rabbit pAb
價格：
在線咨詢
貨號：
KT012119
產(chǎn)品規(guī)格：
100 μL
Applications：
WB/IHC/IF
Species：
H/M/R,H/M/R,H/M/R
購買數(shù)量：
產(chǎn)品詳情
操作說明
注意事項
Product Information
|
Description |
EIF2S2 rabbit polyclonal |
|
Protein full name |
Eukaryotic translation initiation factor 2 subunit 2 |
|
Synonyms |
eIF-2-beta, Eif2s2, Eukaryotic translation initiation factor 2 subunit beta |
|
Immunogen |
Recombinant protein corresponding to Mouse EIF2S2 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm |
|
Predicted MW. |
38 kDa |
|
Observed MW. |
50 kDa |
|
Uniprot ID |
P20042, Q99L45 |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
lung, colon, small intestine |
|
IHC |
Human, Mouse, Rat |
1: 1000-1: 2000 |
colon, lung, placenta |
|
IF |
Human, Mouse, Rat |
1: 500-1: 3000 |
colon, brain |
Background
Eukaryotic translation initiation factor 2 (eIF2) is composed of three subunits, eIF2 alpha, eIF2 beta (EIF2S2), and eIF2 gamma, which are present in equal molar amounts. eIF2 beta plays a central role in the maintenance of what is generally considered a rate-limiting step in mRNA translation. In the early steps of protein synthesis, eIF2 beta binds GTP and Met-tRNA and transfers Met-tRNA to the 40S ribosomal subunit. At the end of the initiation process, GTP bound to eIF2 beta is hydrolyzed to GDP and the eIF2/GDP complex is released from the ribosome. The exchange of GDP bound to eIF2 beta for GTP is a prerequisite to binding Met-tRNA and is mediated by eIF2 beta, which recycles the eIF2 complex for another round of initiation.
Images
|
|
Western blot analysis of EIF2S2 (GB111135) at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded human colon using EIF2S2 (GB111135) at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded rat lung using EIF2S2 (GB111135) at dilution of 1: 2000 |
|
|
Immunohistochemistry analysis of paraffin-embedded rat placenta using EIF2S2 (GB111135) at dilution of 1: 1000 |
|
|
Immunofluorescent analysis of paraffin-embedded human colon using EIF2S2 (GB111135) at dilution of 1: 1000 |
|
|
Immunofluorescent analysis of paraffin-embedded rat brain using EIF2S2 (GB111135) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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合肥萬物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學(xué)科技園A401室